Protein switch that kills deadly water-based pathogens discovered

TORONTO - Scientists have discovered a protein switch that will help kill deadly water-based pathogens like E. coli and make for safer drinking water.

Zongchao Jia, a professor of biochemistry at Queen’s University, and post-doctoral student Jimin Zheng, discovered exactly how the AceK protein acts as a switch in some bacteria to bypass the energy-producing cycle.

This bypassing allows dangerous bacteria like E. coli and salmonella to go into a survival mode and adapt to low-nutrient environments, such as water.

The unique feature of this discovery is that the switching on and off take place in the same location of the protein. Normally, these two opposing activities would happen in two different ‘active sites’.

“From a protein function point of view, this is unique and has never been discovered anywhere else,” says Jia.

The discovery opens the door for scientists to identify a molecule that can keep the bypass switch from turning on so bacteria will die in water.

As a result, drinking water would be cleaner and the incident of water bacterial contamination could be reduced.

“While other organisms cannot survive without nutrients, the bypass controlled by AceK allows the bacteria to live and cause health problems,” says Jia.

Conversely, discovering a molecule to keep the bypass switch turned on could produce a supply of the bacteria which biotechnology companies use to produce compounds such as insulin.

Instead of using glucose in the fermenting process, companies could use less nutritional and cheaper acetate.

The cost difference would be tremendous and the process would produce less carbon dioxide making the process much more environmentally friendly, said a Queen’s release.

“So we haven’t found a cure to stop diseases like E. coli water contamination, but we’ve provided a template for people to design a molecule that will disable its ability to survive in water,” says Jia.

“It’s like we have discovered how a lock works and now all we need is to design a key,” concluded Jia.

These findings have been published in Nature.